Word count: 3000 words

Objectives to cover:

  • Introduction: Kainth (Pyrus pashia) is a wild pear rich in bioactive polyphenols with promising nutraceutical value.

  • Polyphenolic Compounds: These plant-based antioxidants provide diverse health benefits, including anti-inflammatory and disease-preventive effects.

  • Extraction Significance: Efficient extraction techniques are essential for isolating and preserving phytochemicals for research and industry.

  • Solvent Extraction: A conventional method using organic or aqueous solvents to dissolve and recover target bioactives.

  • Ultrasound-Assisted Extraction: Uses acoustic cavitation to enhance solvent penetration and improve polyphenol yield.

  • Microwave-Assisted Extraction: A rapid, energy-efficient, and eco-friendly technique utilizing microwave energy for extraction.

  • Method Comparison: Evaluates yield, purity, time efficiency, and environmental impact of different extraction methods.

  • Polyphenol Quantification: Employs analytical tools like HPLC, UV–Vis spectroscopy, and Folin-Ciocalteu assays for measurement.

  • Conclusion: Selecting the optimal extraction method supports functional food development and nutraceutical innovation.

Reference:  APA style